Development, Validation and Application of a High Sensitivity On-line Extraction LC-MS/MS Method for the Determination of a Parent Molecule and its Metabolites M1 and M2 in Human Plasma
Published: 16 May 2013
Mohammed Abrar, John Allanson, Hayley Hawthorne and Kevin Holmes
Poster Presentation at Joint JPAG/DMDG Symposium: New approaches in bioanalysis and analytical technologies, London

The objective of this study was to validate the analytical method for the determination of a parent compound and its main metabolites (M1 and M2) in human plasma, over a concentration range of 0.1 to 20 pg/mL (parent) and 0.25 to 20 pg/mL (M1 and M2).  A sample aliquot volume of 500 µL was used.  This assay concentration range was required due to the high potency and hence low therapeutic doses of the parent compound administered.  The typical Cmax was expected to be in the low pg/mL levels.